ASRGL1 Polyklonaler Antikörper

ASRGL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 11400-1-AP

Synonyme

CRASH, Beta-aspartyl-peptidase, ASRGL 1, Asparaginase-like protein 1, ALP1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11400-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11400-1-AP (ASRGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11400-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11400-1-AP (ASRGL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11400-1-AP

  IP result of anti-ASRGL1 (IP:11400-1-AP, 4ug; Detection:11400-1-AP 1:500) with HeLa cells lysate 3200ug.

• 

  IHC staining of human gliomas using 11400-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11400-1-AP (ASRGL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11400-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11400-1-AP (ASRGL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

11400-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ASRGL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASRGL1 fusion protein Ag1955
• Vollständiger Name: asparaginase like 1
• Berechnetes Molekulargewicht: 308 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-40 kDa, 20-25 kDa, 15-19 kDa
• GenBank-Zugangsnummer: BC021295
• Gene symbol: ASRGL1
• Gene ID (NCBI): 80150
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Asparaginase-like protein 1 (ASRGL1) is also named as ALP, CRASH and belongs to the Ntn-hydrolase family. ASRGL1 has both L-asparaginase and beta-aspartyl peptidase activity. It may be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. ASRGL1 has 2 isoforms produced by alternative splicing with the MW of 32 kDa and 19 kDa. Two lower molecular weight bands (23 and 15 kDa) can be detected corresponding to the processed α and β subunits (PMID: 27106100).

Publikationen

Species	Application	Title
human		Cell Metab; As Extracellular Glutamine Levels Decline, Asparagine Becomes an Essential Amino Acid.; Authors - Natalya N Pavlova
mouse	WB	Nat Chem Biol; PMID: 28671681; Authors - Nunzio Iraci
mouse	IF	Hum Mol Genet; A missense mutation in the ASRGL1 gene is involved in causing autosomal recessive retinal degeneration.; Authors - Pooja Biswas
cat	IHC	Front Cell Dev Biol; Oocyte Meiotic Competence in the Domestic Cat Model: Novel Roles for Nuclear Proteins BRD2 and NPM1.; Authors - Daniela R Chavez
human	WB	Front Oncol; ASRGL1 Correlates With Immune Cell Infiltration in Hepatocellular Carcinoma and Can Serve as a Prognostic Biomarker.; Authors - Cailin Xue
mouse	WB	Inflammation; Ghrelin Fights Against Titanium Particle-Induced Inflammatory Osteolysis Through Activation of β-Catenin Signaling Pathway.; Authors - Ruize Qu