ASL Polyklonaler Antikörper

ASL Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 16645-1-AP

Synonyme

argininosuccinate lyase, Arginosuccinase, ASAL, ASL

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Galerie der Validierungsdaten

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  WB analysis using 16645-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16645-1-AP (ASL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 16645-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16645-1-AP (ASL Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16645-1-AP (ASL Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 16645-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16645-1-AP (ASL Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Mauslebergewebe, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

16645-1-AP bindet in WB, IHC, ELISA ASL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASL fusion protein Ag10007
• Vollständiger Name: argininosuccinate lyase
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC008195
• Gene symbol: ASL
• Gene ID (NCBI): 435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)

Publikationen

Species	Application	Title
mouse	WB	Mol Metab; SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.; Authors - Wenxiang Zhang
human,mouse	WB	Cell Discov; TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential; Authors - Jing He
human	WB,IHC	J Proteome Res; Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.; Authors - Yin Cao