ARPC4 Polyklonaler Antikörper

ARPC4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10930-1-AP

Synonyme

ARC20, Arp2/3 complex 20 kDa subunit, ARPC4, p20 Arc

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 10930-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10930-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10930-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10930-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 10930-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 10930-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lymph using 10930-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10930-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of 4T1 using 10930-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 10930-1-AP (ARPC4 antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10930-1-AP (ARPC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10930-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 10930-1-AP (ARPC4 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, 4T1-Zellen, humanes Hirngewebe, humanes Plazenta-Gewebe, Maus-Cerebellum-Gewebe, Maus-Dünndarmgewebe, MCF-7-Zellen, Rattenlymphgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Hodengewebe, humanes Lungenkarzinomgewebe, humanes Urothelkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

10930-1-AP bindet in WB, IHC, ELISA ARPC4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARPC4 fusion protein Ag1366
• Vollständiger Name: actin related protein 2/3 complex, subunit 4, 20kDa
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 19-25 kDa
• GenBank-Zugangsnummer: BC012596
• Gene symbol: ARPC4
• Gene ID (NCBI): 10093
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ARP2/3 complex consists of seven subunits: two actin-related proteins (ARP2 and ARP3) and five ARP2/3 complex polypeptides (ARPC1B and ARPC2-5). ARPC4 is a core subunit of the actin-related protein (ARP2/3) complex, which catalyzes the formation of F-actin networks. ARPC4 is essential for the structural integrity of the ARP2/3 complex function. Mutations in ARPC4 cause Developmental delay, language impairment, and ocular abnormalities (DEVLO)(PMID: 35047857).

Publikationen

Species	Application	Title
human	WB	Life Sci Alliance; A high-throughput two-cell assay for interrogating inhibitory signaling pathways in T cells; Authors - Sumana Sharma
human	WB	Biochim Biophys Acta Proteins Proteom; Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis.; Authors - Guoxuan Qin
human	WB	Drug Resist Updat; Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane; Authors - Xin Zhang