BBS3 Polyklonaler Antikörper

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• KD/KO Validated

BBS3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 12676-1-AP

Synonyme

ARL6, Bardet-Biedl syndrome 3 protein, ADP-ribosylation factor-like protein 6, ADP ribosylation factor like 6

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Galerie der Validierungsdaten

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  WB analysis of rat brain using 12676-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12676-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 12676-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12676-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12676-1-AP (BBS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 12676-1-AP

  IP result of anti-BBS3 (IP:12676-1-AP, 3ug; Detection:12676-1-AP 1:600) with rat brain tissue lysate 5200ug.

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  IHC staining of human kidney using 12676-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12676-1-AP (BBS3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12676-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12676-1-AP (BBS3 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of hTERT-RPE1 cells using 12676-1-AP

  IF result (cytoplasm and the base of cilia stain) of anti-BBS3 (12676-1-AP; 1:50) with hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.

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  IF Staining of MDCK using 12676-1-AP

  Immunofluorescent analysis of MDCK cells using 12676-1-AP (BBS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, HEK-293T-Zellen, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen, MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

12676-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA BBS3 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BBS3 fusion protein Ag3363
• Vollständiger Name: ADP-ribosylation factor-like 6
• Berechnetes Molekulargewicht: 186 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC024239
• Gene symbol: ARL6/BBS3
• Gene ID (NCBI): 84100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factor-like protein 6 (ARL6), belongs to a small GTPase superfamily, is involved in membrane protein trafficking at the base of the ciliary organelle. ARL6 is also named Bardet-Biedl syndrome 3 protein, because defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3), which is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. ARL6 can interact with many ARL6 interacting proteins and form BBSome complex with other BBS proteins including BBS1, BBS2, BBS4 and so on.

Publikationen

Species	Application	Title
mouse	WB,IF	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
human	WB	Proc Natl Acad Sci U S A; ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.; Authors - Humbert Melissa C MC ; KD Validated
mouse	WB	PLoS Genet; BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.; Authors - Ying Hsu
human	WB	J Cell Sci; Basal body proteins regulate Notch signaling via endosomal trafficking.; Authors - Carmen C Leitch
mouse	IHC,IF	Bone; Bardet-Biedl Syndrome 3 regulates development of cranial base midline structures.; Authors - Makiri Kawasaki ; KO Validated
human	IF	Biol Open; ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.; Authors - Sayaka Fujisawa