ARL3 Polyklonaler Antikörper

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• KD/KO Validated

ARL3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 10961-1-AP

Synonyme

ADP-ribosylation factor-like protein 3, ADP ribosylation factor like 3

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Galerie der Validierungsdaten

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  WB analysis using 10961-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10961-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10961-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10961-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10961-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10961-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10961-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10961-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 10961-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 10961-1-AP (ARL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10961-1-AP (ARL3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10961-1-AP (ARL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10961-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10961-1-AP (ARL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of IMCD3 cells using 10961-1-AP

  IF result of 10961-1-AP (anti-ARL3,Green) in ciliated IMCD3 cells by Dr. Seongjin Seo. (Red is the primary cilium, and blue is the nucleus).

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  IF Staining of hTERT-RPE1 using 10961-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MDCK using 10961-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using ARL3 antibody (10961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, humanes Hirngewebe, Maushodengewebe, PC-3-Zellen, Rattenhirngewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MDCK-Zellen, hTERT-RPE1-Zellen, IMCD3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Produktinformation

10961-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ARL3 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL3 fusion protein Ag1406
• Vollständiger Name: ADP-ribosylation factor-like 3
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa, 25 kDa
• GenBank-Zugangsnummer: BC009841
• Gene symbol: ARL3
• Gene ID (NCBI): 403
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
human	WB,IF	Nat Commun; Spatial cycles mediated by UNC119 solubilisation maintain Src family kinases plasma membrane localisation.; Authors - Antonios D Konitsiotis
mouse	WB,IF, IP	Nat Commun; Arl3 and LC8 regulate dissociation of dynactin from dynein.; Authors - Mingyue Jin ; KD Validated
mouse	WB	Nat Commun; Ciliary membrane proteins traffic through the Golgi via a Rabep1/GGA1/Arl3-dependent mechanism.; Authors - Hyunho Kim ; KD Validated
human	WB	Proc Natl Acad Sci U S A; ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.; Authors - Humbert Melissa C MC
human	IF	Dev Cell; The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK.; Authors - Louise A Stephen