ARL13B Polyklonaler Antikörper

ARL13B Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Guinea Pig / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30332-1-AP

Synonyme

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8

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Galerie der Validierungsdaten

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  WB analysis using 30332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30332-1-AP (ARL13B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The second antibody is used against guinea pigs.

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  IP experiment of L02 using 30332-1-AP

  IP result of anti-ARL13B(IP:30332-1-AP, 4ug; Detection:30332-1-AP 1:4000) with L02 cells lysate 3080 ug.

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  IF Staining of hTERT-RPE1 using 30332-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (30332-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, K-562-Zellen, L02-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IF: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30332-1-AP bindet in WB, IP, IF, ELISA ARL13B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Guinea Pig / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARL13B fusion protein Ag12015
• Vollständiger Name: ADP-ribosylation factor-like 13B
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: BC094725
• Gene symbol: ARL13B
• Gene ID (NCBI): 200894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.