• Featured Product
  • KD/KO Validated

RhoGDI Polyklonaler Antikörper

RhoGDI Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10509-1-Ig

Synonyme

aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HL-60-Zellen, Maus-Pankreasgewebe
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10509-1-Ig bindet in WB, IHC, ELISA RhoGDI und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RhoGDI fusion protein Ag0791
Vollständiger Name Rho GDP dissociation inhibitor (GDI) alpha
Berechnetes Molekulargewicht 28 kDa
Beobachtetes Molekulargewicht 28 kDa
GenBank-ZugangsnummerBC005851
Gene symbol RhoGDI
Gene ID (NCBI) 396
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.

Protokolle

Produktspezifische Protokolle
WB protocol for RhoGDI antibody 10509-1-IgProtokoll herunterladen
IHC protocol for RhoGDI antibody 10509-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cancer Res

miR-483-5p Promotes Invasion and Metastasis of Lung Adenocarcinoma by Targeting RhoGDI1 and ALCAM.

Authors - Qiancheng Song
  • KD Validated
humanWB

Oncotarget

Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.

Authors - Hao Bo
mouseWB

Sci Rep

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.

Authors - Dongli Liang
humanWB

J Proteome Res

Quantitative analysis of the detergent-insoluble brain proteome in frontotemporal lobar degeneration using SILAC internal standards.

Authors - Seyfried Nicholas T NT
humanWB

J Exp Clin Cancer Res

Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway.

Authors - Yu Lian
humanWB

PLoS One

Comparative expression profiling of distinct T cell subsets undergoing oxidative stress.

Authors - Lichtenfels Rudolf R

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Udesh (Verified Customer) (11-26-2023)

Worked well at 1:2000 for Glioblastoma cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: GBM