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RhoGDI Polyklonaler Antikörper
RhoGDI Polyklonal Antikörper für WB, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10509-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Jurkat-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HL-60-Zellen, Maus-Pankreasgewebe |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunhistochemie (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 11 publications below |
IHC | See 1 publications below |
Produktinformation
10509-1-Ig bindet in WB, IHC, ELISA RhoGDI und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | RhoGDI fusion protein Ag0791 |
Vollständiger Name | Rho GDP dissociation inhibitor (GDI) alpha |
Berechnetes Molekulargewicht | 28 kDa |
Beobachtetes Molekulargewicht | 28 kDa |
GenBank-Zugangsnummer | BC005851 |
Gene symbol | RhoGDI |
Gene ID (NCBI) | 396 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-A-Reinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for RhoGDI antibody 10509-1-Ig | Protokoll herunterladen |
IHC protocol for RhoGDI antibody 10509-1-Ig | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cancer Res miR-483-5p Promotes Invasion and Metastasis of Lung Adenocarcinoma by Targeting RhoGDI1 and ALCAM.
| ||
Oncotarget Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. | ||
Sci Rep Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module. | ||
J Proteome Res Quantitative analysis of the detergent-insoluble brain proteome in frontotemporal lobar degeneration using SILAC internal standards. | ||
J Exp Clin Cancer Res Long noncoding RNA AFAP1-AS1 acts as a competing endogenous RNA of miR-423-5p to facilitate nasopharyngeal carcinoma metastasis through regulating the Rho/Rac pathway. | ||
PLoS One Comparative expression profiling of distinct T cell subsets undergoing oxidative stress. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Udesh (Verified Customer) (11-26-2023) | Worked well at 1:2000 for Glioblastoma cells
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