ARHGAP15 Polyklonaler Antikörper

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• KD/KO Validated

ARHGAP15 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11940-1-AP

Synonyme

BM-046, BM046, BM-030, BM-024, BM 046

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Galerie der Validierungsdaten

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  WB analysis using 11940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11940-1-AP (ARHGAP15 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 11940-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11940-1-AP (ARHGAP15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11940-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11940-1-AP (ARHGAP15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 11940-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ARHGAP15 antibody (11940-1-AP, Clone: 50-55 kDa ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11940-1-AP bindet in WB, IHC, IF/ICC, ELISA ARHGAP15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGAP15 fusion protein Ag2538
• Vollständiger Name: Rho GTPase activating protein 15
• Berechnetes Molekulargewicht: 475 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC016701
• Gene symbol: ARHGAP15
• Gene ID (NCBI): 55843
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARHGAP15 (Rho GTPase Activating Protein 15) is a Rac1-specific GAP. Diseases associated with ARHGAP15 include Diverticulitis and Meier-Gorlin Syndrome 2. Among its related pathways are Signaling by Rho GTPases and RAC2 GTPase cycle. ARHGAP15 deregulation is implicated in many abnormalities (PMID: 29867200). ARHGAP15 decreased in glioma, which promoted the aggressive phenotypes of glioma cells through the activation of Rac19. Intronic mutation of ARHGAP15 is associated with diverticular disease. In addition, ARHGAP15 was a prognosis-related biomarker for pancreatic ductal adenocarcinoma (PMID: 28979141).

Publikationen

Species	Application	Title
mouse	IF	Front Cell Dev Biol; Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area.; Authors - Kang Guo
human	WB,IHC	Cancer Sci; Forkhead box P3 regulates ARHGAP15 expression and affects migration of glioma cells through the Rac1 signaling pathway.; Authors - Zhen Sun ; KD Validated
mouse	WB	J Neurosci; Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation.; Authors - Cheng-Yi Yang