ARHGAP1 Polyklonaler Antikörper

ARHGAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11169-1-AP

Synonyme

p50-RhoGAP, p50rhoGAP, p50 RhoGAP, GTPase-activating protein rhoGAP, CDC42GAP

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Galerie der Validierungsdaten

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  WB analysis of RAW 264.7 using 11169-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 11169-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human duodenum using 11169-1-AP

  human duodenum tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human pancreas using 11169-1-AP

  human pancreas tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11169-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11169-1-AP (ARHGAP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 11169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 11169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11169-1-AP (ARHGAP1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 11169-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11169-1-AP (ARHGAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11169-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11169-1-AP (ARHGAP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, humanes Herzgewebe, humanes Pankreasgewebe, humanes Zwölffingerdarm-Gewebe, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11169-1-AP bindet in WB, IHC, IF/ICC, ELISA ARHGAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARHGAP1 fusion protein Ag1651
• Vollständiger Name: Rho GTPase activating protein 1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC018118
• Gene symbol: ARHGAP1
• Gene ID (NCBI): 392
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.