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  • KD/KO Validated

ARFGAP1 Polyklonaler Antikörper

ARFGAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13571-1-AP

Synonyme

ARF1GAP, ARF1-directed GTPase-activating protein, ARF1 GAP, ARF GAP 1, ADP-ribosylation factor GTPase-activating protein 1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-3-Zellen, HeLa-Zellen, humanes Hirngewebe
Erfolgreiche IPMaushodengewebe
Erfolgreiche Detektion in IHChumanes Prostatakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13571-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ARFGAP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Hausschwein, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ARFGAP1 fusion protein Ag4468
Vollständiger Name ADP-ribosylation factor GTPase activating protein 1
Berechnetes Molekulargewicht 414 aa, 46 kDa
Beobachtetes Molekulargewicht 46 kDa, 50 kDa
GenBank-ZugangsnummerBC028233
Gene symbol ARFGAP1
Gene ID (NCBI) 55738
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein coats deform flat lipid membranes into buds and capture membrane proteins to form transport vesicles. The assembly-disassembly cycle of the COPI coat on Golgi membranes is coupled to the GTP-GDP cycle of the small G protein ARF1. ARFGAP1 contributes to vesicle budding by increasing the GTPase activity of ARF1, because it promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. for ARFGAP1 may has a role in regulating the GTPase activity and neuronal toxicity of LRRK2; reciprocally, LRRK2 phosphorylates ArfGAP1 and is required for ArfGAP1 neuronal toxicity.

Protokolle

Produktspezifische Protokolle
WB protocol for ARFGAP1 antibody 13571-1-APProtokoll herunterladen
IHC protocol for ARFGAP1 antibody 13571-1-APProtokoll herunterladen
IF protocol for ARFGAP1 antibody 13571-1-APProtokoll herunterladen
IP protocol for ARFGAP1 antibody 13571-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
mouse,ratWB,IF

PLoS Genet

GTPase activity and neuronal toxicity of Parkinson's disease-associated LRRK2 is regulated by ArfGAP1.

Authors - Klodjan Stafa
  • KD Validated
humanWB, IF

FASEB J

ArfGAP1 acts as a GTPase-activating protein for human ADP-ribosylation factor-like 1 protein.

Authors - Hsiang-Pu Feng
  • KD Validated
human,pigWB

Vet Microbiol

ARFGAP1 binds to classical swine fever virus NS5A protein and enhances CSFV replication in PK-15 cells.

Authors - Liang Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Boyan (Verified Customer) (10-27-2021)

This antibody clearly label the Golgi localisation of endogenous ArfGAP1, and also gives a clean signal in WB.

  • Applications: Western Blot, Immunofluorescence
FH

Cristian (Verified Customer) (04-17-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa
ARFGAP1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:13571-1-AP)