ARF6-Specific Polyklonaler Antikörper

ARF6-Specific Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 20225-1-AP

Synonyme

ADP ribosylation factor 6, ARF6, ARF6-Specific

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Galerie der Validierungsdaten

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  WB analysis using 20225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20225-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20225-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20225-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 20225-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20225-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20225-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, HEK-293-Zellen, humanes Lebergewebe, Mauslebergewebe, MCF-7-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Herzgewebe, humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

20225-1-AP bindet in WB, IHC, IF, ELISA ARF6-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ADP-ribosylation factor 6
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: NM_001663
• Gene symbol: ARF6
• Gene ID (NCBI): 382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
human	WB	Int J Biol Sci; Platelet-derived extracellular vesicles aggravate septic acute kidney injury via delivering ARF6; Authors - Xun Lu ; KD Validated
human	WB	Arterioscler Thromb Vasc Biol; BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.; Authors - Lin Sisi S
mouse	WB,IHC	Int J Mol Sci; Distribution of Signal Peptides in Microvesicles from Activated Macrophage Cells; Authors - Kenji Ono
mouse		Sci Rep; Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.; Authors - Ermes Candiello
human	IF	PLoS One; TNF Stimulates Nuclear Export and Secretion of IL-15 by Acting on CRM1 and ARF6.; Authors - Ouyang Suidong S