ARF3 Polyklonaler Antikörper

ARF3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10800-1-AP

Synonyme

ADP-ribosylation factor 3, ADP ribosylation factor 3

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 10800-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10800-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10800-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse cerebellum using 10800-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10800-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10800-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10800-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10800-1-AP (ARF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10800-1-AP (ARF3 antibody) at dilution of 1:10 (under 10x lens).

• 

  IHC staining of human pancreas using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10800-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10800-1-AP (ARF3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 10800-1-AP

  Immunofluorescent analysis of HepG2 cells using 10800-1-AP (ARF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10800-1-AP

  Immunofluorescent analysis of HepG2 cells using 10800-1-AP (ARF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe, Maus-Cerebellum-Gewebe, Raji-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Pankreasgewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

10800-1-AP bindet in WB, IHC, IF/ICC, ELISA ARF3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARF3 fusion protein Ag1174
• Vollständiger Name: ADP-ribosylation factor 3
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC017565
• Gene symbol: ARF3
• Gene ID (NCBI): 377
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562). This antibody can bind ARFs for the close sequences.

Publikationen

Species	Application	Title
mouse	WB,IHC	Development; PFN4 is required for manchette development and acrosome biogenesis during mouse spermiogenesis; Authors - Naila Umer