Amphiregulin Monoklonaler Antikörper

Amphiregulin Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

1A1G9

Kat-Nr. : 66433-1-Ig

Synonyme

amphiregulin, AR, AREG, AREGB, CRDGF, SDGF

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Galerie der Validierungsdaten

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  WB analysis of A549 using 66433-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66433-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 66433-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66433-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66433-1-Ig (Amphiregulin antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 66433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66433-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66433-1-Ig (Amphiregulin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Hausschwein-Hirngewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

66433-1-Ig bindet in WB, IHC, IF, ELISA Amphiregulin und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Amphiregulin fusion protein Ag8907
• Vollständiger Name: amphiregulin
• Berechnetes Molekulargewicht: 252 aa, 28 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 37 kDa
• GenBank-Zugangsnummer: BC009799
• Gene symbol: AREG
• Gene ID (NCBI): 374
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).

Publikationen

Species	Application	Title
human	IF	Cell Rep Med; Tumor-associated monocytes promote mesenchymal transformation through EGFR signaling in glioma; Authors - Yiyun Chen
human	WB	J Invest Dermatol; LPCAT1 promotes cutaneous squamous cell carcinoma via EGFR-mediated AKT/p38MAPK signaling pathways.; Authors - Yingjian Huang
		Int J Biol Sci; CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17.; Authors - Jie Liu