Apolipoprotein H Polyklonaler Antikörper

Apolipoprotein H Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11892-1-AP

Synonyme

APOH, B2G1, ApolipoproteinH, Apo-H, Apo H

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Galerie der Validierungsdaten

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  WB analysis using 11892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11892-1-AP (Apolipoprotein H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11892-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11892-1-AP (Apolipoprotein H antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human normal colon using 11892-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11892-1-AP (Apolipoprotein H antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 11892-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11892-1-AP (Apolipoprotein H antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11892-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein H antibody (11892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: human normal colon; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

11892-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Apolipoprotein H und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Apolipoprotein H fusion protein Ag2500
• Vollständiger Name: apolipoprotein H (beta-2-glycoprotein I)
• Berechnetes Molekulargewicht: 345 aa, 38 kDa
• Beobachtetes Molekulargewicht: 43-55 kDa
• GenBank-Zugangsnummer: BC026283
• Gene symbol: APOH
• Gene ID (NCBI): 350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins. ApoH is a 54 kDa plasma glycoprotein.

Publikationen

Species	Application	Title
human	IHC	Nat Commun; ATRX, DAXX or MEN1 mutant pancreatic neuroendocrine tumors are a distinct alpha-cell signature subgroup.; Authors - Chang S Chan
human	IP	Neuropharmacology; Anti-β2GPI/β2GPI induces neutrophil extracellular traps formation to promote thrombogenesis via the TLR4/MyD88/MAPKs axis activation.; Authors - Caijun Zha