APOBEC3C Polyklonaler Antikörper

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• KD/KO Validated

APOBEC3C Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10591-1-AP

Synonyme

APOBEC1 like, APOBEC1L, APOBEC3, APOBEC3C, ARDC2, ARDC4, ARP5, bK150C2.3, PBI, Phorbolin I

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10591-1-AP

  WB result of APOBEC3C antibody (10591-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APOBEC3C transfected HEK-293 cells.

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  WB analysis using 10591-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10591-1-AP (APOBEC3C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10591-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10591-1-AP (APOBEC3C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caco-2-Zellen, HEK-293-Zellen, K-562-Zellen, SW480-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

10591-1-AP bindet in WB, IF, IHC, ELISA APOBEC3C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: APOBEC3C fusion protein Ag0886
• Vollständiger Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC011739
• Gene symbol: APOBEC3C
• Gene ID (NCBI): 27350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APOBEC3C(apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3C) is also named as APOBEC1L, PBI and belongs to the cytidine and deoxycytidylate deaminase family.APOBEC3C is related to APOBEC1, a cytidine deaminase responsible for cytidine-to-uridine editing of apolipoprotein B (APOB)mRNA.It can form a dimer(PMID:19074429).

Publikationen

Species	Application	Title
mouse	WB	J Exp Med; APOBEC3A catalyzes mutation and drives carcinogenesis in vivo.; Authors - Emily K Law
human	WB	Nucleic Acids Res; Enterovirus 71 antagonizes the inhibition of the host intrinsic antiviral factor A3G.; Authors - Zhaolong Li
human	IHC	Oncogenesis; Aberrant APOBEC3C expression induces characteristic genomic instability in pancreatic ductal adenocarcinoma.; Authors - Yunzhen Qian
human	IHC	Sci Rep; Expression and subcellular localisation of AID and APOBEC3 in adenoid and palatine tonsils.; Authors - Noriko Seishima
human	WB	J Virol; Infection of Bronchial Epithelial Cells by the Human Adenoviruses A12, B3, and C2 Differently Regulates the Innate Antiviral Effector APOBEC3B.; Authors - Noémie Lejeune
human	WB	Retrovirology; Natural APOBEC3C variants can elicit differential HIV-1 restriction activity.; Authors - Brett D Anderson