Apelin Polyklonaler Antikörper

Apelin Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 11497-1-AP

Synonyme

APLN, APJ endogenous ligand, APEL

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Galerie der Validierungsdaten

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  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11497-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11497-1-AP (Apelin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11497-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Apelin antibody (11497-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Mausherzgewebe, Maushirngewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

11497-1-AP bindet in IHC, IF/ICC, ELISA Apelin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Apelin fusion protein Ag2047
• Vollständiger Name: apelin
• Berechnetes Molekulargewicht: 77 aa, 9 kDa
• GenBank-Zugangsnummer: BC021104
• Gene symbol: Apelin
• Gene ID (NCBI): 8862
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Apelin, isolated from bovine stomach tissue extractsis, is recognized as the endogenous ligand of the angiotensin-like-receptor 1 (APJ), and the human orphan G-protein-coupled receptor. Apelin is widely expressed in various organs such as the heart, lung, kidney, liver, adipose tissue, gastrointestinal tract, brain, adrenal glands, endothelium, and human plasma. Apelin has been found to be a potent stimulator of cardiac contractility and may function in the regulation of the cardiovascular system. Apelin is also known to be involved in the maintenance of INS sensitivity and play an important role in liver disease.

Publikationen

Species	Application	Title
mouse		Sci Adv; Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.; Authors - Jun Seok Son
rat	IHC	Free Radic Biol Med; Apelin-13 induces mitophagy in bone marrow mesenchymal stem cells to suppress intracellular oxidative stress and ameliorate osteoporosis by activation of AMPK signaling pathway; Authors - Liang Chen
mouse		EBioMedicine; Maternal exercise intergenerationally drives muscle-based thermogenesis via activation of apelin-AMPK signaling.; Authors - Jun Seok Son
rat		Front Pharmacol; Apelin-13 Suppresses Neuroinflammation Against Cognitive Deficit in a Streptozotocin-Induced Rat Model of Alzheimer's Disease Through Activation of BDNF-TrkB Signaling Pathway.; Authors - Huaiqing Luo
rat		Neuroscience; Apelin-13 Upregulates BDNF Against Chronic Stress-induced Depression-like Phenotypes by Ameliorating HPA Axis and Hippocampal Glucocorticoid Receptor Dysfunctions.; Authors - Ting-Ting Dai
rat		Neuroscience; The Hippocampus is a Critical Site Mediating Antidepressant-like Activity of Apelin-13 in Rats.; Authors - Zhi-Yong Xiao