APC Polyklonaler Antikörper

APC Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 19782-1-AP

Synonyme

adenomatous polyposis coli, APC, BTPS2, Deleted in polyposis 2.5, DP2, DP2.5, DP3, GS, Protein APC

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Galerie der Validierungsdaten

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  IHC staining of human breast cancer using 19782-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human breast cancer using 19782-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human colon using 19782-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human colon using 19782-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human colon cancer using 19782-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human colon cancer using 19782-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human endometrial cancer using 19782-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human endometrial cancer using 19782-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19782-1-AP (APC Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

19782-1-AP bindet in WB, IHC, ELISA APC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: adenomatous polyposis coli
• Berechnetes Molekulargewicht: 312 kDa
• GenBank-Zugangsnummer: NM_000038
• Gene symbol: APC
• Gene ID (NCBI): 324
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APC, also named as DP2.5, belongs to the adenomatous polyposis coli (APC) family. APC is a tumor suppressor that regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division, and associates with other proteins involved in cell attachment and signaling. APC promotes rapid degradation of CTNNB1 and participates in Wnt signaling as a negative regulator. It plays a critical role in several cellular processes. APC regulates beta-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. APC activity is correlated with its phosphorylation state. Defects in APC are a cause of familial adenomatous polyposis (FAP) which includes also Gardner syndrome (GS). Defects in APC are a cause of hereditary desmoid disease (HDD) which also known as familial infiltrative fibromatosis (FIF). Defects in APC are a cause of medulloblastoma (MDB) which is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Defects in APC are a cause of mismatch repair cancer syndrome (MMRCS) which also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1).

Publikationen

Species	Application	Title
human	WB	Mol Oncol; Norepinephrine-CREB1-miR-373 axis promotes progression of colon cancer.; Authors - Jia Han
human	WB	Biomed Pharmacother; Glycogen phosphorylase B promotes ovarian cancer progression via Wnt/β-catenin signaling and is regulated by miR-133a-3p.; Authors - Yang Zhou
human	WB	World J Gastroenterol; MiR-205 mediated APC regulation contributes to pancreatic cancer cell proliferation.; Authors - Rui-Feng Qin
human	WB	Cancer Med; MiR-26b suppresses hepatocellular carcinoma development by negatively regulating ZNRD1 and Wnt/β-catenin signaling.; Authors - Xiaobo Hu
human	WB	J Bone Oncol; BAIAP2L2 promotes the proliferation, migration and invasion of osteosarcoma associated with the Wnt/β-catenin pathway; Authors - Hongting Guo
mouse	WB	Evid Based Complement Alternat Med; Intervention Mechanism of Hunag-Lian Jie-Du Decoction on Canonical Wnt/β-Catenin Signaling Pathway in Psoriasis Mouse Model.; Authors - Xuesong Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brianna (Verified Customer) (12-13-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mouse tissue

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