AP3B1 Polyklonaler Antikörper

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• KD/KO Validated

AP3B1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 13384-1-AP

Synonyme

ADTB3, ADTB3A, AP 3 complex subunit beta 1, AP3B1, Beta 3A adaptin, HPS, HPS2, PE

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Galerie der Validierungsdaten

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  WB analysis using 13384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13384-1-AP (AP3B1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 13384-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13384-1-AP (AP3B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 13384-1-AP

  IP result of anti-AP3B1 (IP:13384-1-AP, 3ug; Detection:13384-1-AP 1:500) with COLO 320 cells lysate 2500ug.

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  IHC staining of rat brain using 13384-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 13384-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13384-1-AP (AP3B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, Maus-Thymusgewebe, SKOV-3-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IF: See 2 publications below

Produktinformation

13384-1-AP bindet in WB, IP, IF, IHC, ELISA AP3B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP3B1 fusion protein Ag4225
• Vollständiger Name: adaptor-related protein complex 3, beta 1 subunit
• Berechnetes Molekulargewicht: 1094 aa, 121 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC038444
• Gene symbol: AP3B1
• Gene ID (NCBI): 8546
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP3B1 is the 140-kDa β3A subunit of the adaptor-related protein complex-3 (AP-3), a ubiquitous heterotetrameric complex that is localized to the trans-Golgi network and endosomes and is involved in protein trafficking to lysosomes or specialized endosomal-lysosomal organelles (PMID: 9182526; 9545220). This complex is composed of two larger subunits (δ and β3A or β3B), a medium subunit (μ3A or μ3B), and a small subunit (σ3A or σ3B). The absence of the β3A subunit (AP3B1) results in the loss of stability of AP3 and leads to degradation of μ3A, to which β3A is directly bound, while the other subunits are variably affected (PMID: 16507770). AP3B1 contains three main domains: the N-terminal head domain, the hinge, and the C-terminal ear domain. It has been reported as a target of IP(7)-mediated pyrophosphorylation (PMID: 19934039). Defects in AP3B1 are the cause of Hermansky-Pudlak syndrome type 2 (HPS2) (PMID: 10024875; 16507770).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Misrouting of v-ATPase subunit V0a1 dysregulates lysosomal acidification in a neurodegenerative lysosomal storage disease model.; Authors - Maria B Bagh
human	WB	Cell Rep; Modeling of Fibrotic Lung Disease Using 3D Organoids Derived from Human Pluripotent Stem Cells.; Authors - Alexandros Strikoudis ; KO Validated
	WB	EMBO Rep; BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters.; Authors - Ashari R Kannangara
human	WB	J Neurosci; Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1.; Authors - Gokhale Avanti A
human	WB,IF	J Virol; Matrix Proteins of Nipah and Hendra Viruses Interact with Beta Subunits of AP-3 Complexes.; Authors - Weina Sun ; KD Validated
human	WB,IF	J Biol Chem; BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.; Authors - Bultema Jarred J JJ ; KD Validated

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