ANKRD16 Polyklonaler Antikörper

ANKRD16 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24334-1-AP

Synonyme

ANKRD16, ankyrin repeat domain 16

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 24334-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24334-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 24334-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24334-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24334-1-AP (ANKRD16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 24334-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24334-1-AP (ANKRD16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Mauslebergewebe, PC-3-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24334-1-AP bindet in WB, IF,ELISA ANKRD16 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANKRD16 fusion protein Ag19369
• Vollständiger Name: ankyrin repeat domain 16
• Berechnetes Molekulargewicht: 361 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC062346
• Gene symbol: ANKRD16
• Gene ID (NCBI): 54522
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANKRD16, a vertebrate-specific protein that contains ankyrin repeats, binds directly to the catalytic domain of AlaRS. Serine that is misactivated by AlaRS is captured by the lysine side chains of ANKRD16, which prevents the charging of serine adenylates to tRNAAla and precludes serine misincorporation in nascent peptides. The deletion of Ankrd16 in the brains of Aarssti/sti mice causes widespread protein aggregation and neuron loss (PMID: 29769718).