ANKRD1 Polyklonaler Antikörper

ANKRD1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Rezensionen (1)

Kat-Nr. : 11427-1-AP

Synonyme

ALRP, ANKRD1, C 193, C193, Cardiac ankyrin repeat protein, CARP, CVARP, HA1A2, MCARP

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Galerie der Validierungsdaten

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  WB analysis using 11427-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11427-1-AP (ANKRD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 11427-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11427-1-AP (ANKRD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

11427-1-AP bindet in WB, IF, IP, RIP, ELISA ANKRD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANKRD1 fusion protein Ag1970
• Vollständiger Name: ankyrin repeat domain 1 (cardiac muscle)
• Berechnetes Molekulargewicht: 319 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC018667
• Gene symbol: ANKRD1
• Gene ID (NCBI): 27063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression. This is a rabbit polyclonal antibody produced by the full-length ANKRD1 of human origin

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Metabolic control of YAP and TAZ by the mevalonate pathway.; Authors - Giovanni Sorrentino
mouse	IF	Nat Commun; YAP1/TAZ drives ependymoma-like tumour formation in mice.; Authors - Noreen Eder
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; Glucocorticoid receptor signalling activates YAP in breast cancer.; Authors - Giovanni Sorrentino
		Hum Mol Genet; Exploiting The CRISPR/CAS9 System to Study Alternative Splicing In Vivo: Application to Titin.; Authors - Karine Charton
mouse	IF	Cell Death Dis; The histone code reader Spin1 controls skeletal muscle development.; Authors - Holger Greschik

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Luigi (Verified Customer) (11-29-2018)

• Applications: Immunoprecipitation,
• Primary Antibody Dilution: 5 ug
• Cell Tissue Type: Fibroblast

ANKRD1 Antibody Immunoprecipitation, validation (5 ug dilution) in Fibroblast (Cat no:11427-1-AP)