ANGPTL2 Polyklonaler Antikörper

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• KD/KO Validated

ANGPTL2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 12316-1-AP

Synonyme

angiopoietin like 2, Angiopoietin like protein 2, Angiopoietin related protein 2, ANGPTL2, ARP2, HARP

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Galerie der Validierungsdaten

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  WB analysis of mouse small intestine using 12316-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12316-1-AP

  mouse heart tissue was subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 12316-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 12316-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12316-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12316-1-AP (ANGPTL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12316-1-AP

  IP result of anti-ANGPTL2 (IP:12316-1-AP, 4ug; Detection:12316-1-AP 1:500) with mouse testis tissue lysate 4800ug.

• 

  IHC staining of human colon cancer using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 12316-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12316-1-AP (ANGPTL2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, 3T3-L1-Zellen, Maus-Dünndarmgewebe, Maushodengewebe, Mausmilzgewebe
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Herzgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Produktinformation

12316-1-AP bindet in WB, IHC, IF, IP, ELISA ANGPTL2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ANGPTL2 fusion protein Ag2969
• Vollständiger Name: angiopoietin-like 2
• Berechnetes Molekulargewicht: 493 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57-64 kDa
• GenBank-Zugangsnummer: BC012368
• Gene symbol: ANGPTL2
• Gene ID (NCBI): 23452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ANGPTL2 (angiopoietin-like 2), a member of the Angptl protein family, is predominantly secreted from adipose tissue and the heart. ANGPTL2 consists of an N-terminus with a conserved coiled-coil domain, two glycosylation sites and a C-terminus with a conserved fibrinogen-like domain. It is a key adipocyte-derived inflammatory mediator that links obesity to systemic INS resistance. ANGPTL2 may play an important role in the pathogenesis of diabetic glomerulopathy.

Publikationen

Species	Application	Title
rat	WB	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
human	IHC	World J Gastroenterol; Leukocyte immunoglobulin-like receptor B2 overexpression as a promising therapeutic target and noninvasive screening biomarker for colorectal cancer; Authors - Qian-Qian Wang
human	IHC	Biomolecules; Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods; Authors - Haofuzi Zhang
rat	WB	Sci Rep; PET-CT and RNA sequencing reveal novel targets for acupuncture-induced lowering of blood pressure in spontaneously hypertensive rats.; Authors - Jing Li
human,mouse	IHC,WB	J Periodontol; Angiopoietin-like protein 2 deficiency promotes periodontal inflammation and alveolar bone loss.; Authors - Chenxi Jiang
human	WB	PLoS One; Angiopoietin-like protein 2 regulates Porphyromonas gingivalis lipopolysaccharide-induced inflammatory response in human gingival epithelial cells.; Authors - Tasuku Ohno ; KD Validated