AMBRA1 Polyklonaler Antikörper

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• KD/KO Validated

AMBRA1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 13762-1-AP

Synonyme

WDR94, KIAA1736, DDB1- and CUL4-associated factor 3, DCAF3, Activating molecule in BECN1-regulated autophagy protein 1

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 13762-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13762-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 13762-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 13762-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13762-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13762-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13762-1-AP (AMBRA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 13762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13762-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13762-1-AP (AMBRA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13762-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMBRA1 antibody (13762-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HepG2-Zellen, HT-1080.Zellen, humanes Hirngewebe, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

13762-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA AMBRA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AMBRA1 fusion protein Ag4735
• Vollständiger Name: autophagy/beclin-1 regulator 1
• Berechnetes Molekulargewicht: 1208 aa, 133 kDa
• Beobachtetes Molekulargewicht: 130-135 kDa
• GenBank-Zugangsnummer: BC045609
• Gene symbol: AMBRA1
• Gene ID (NCBI): 55626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. AMBRA1 regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation. This antibody works well in WB, IHC and IF application.

Publikationen

Species	Application	Title
human	WB,IHC	Nature; CRL4AMBRA1 is a master regulator of D-type cyclins.; Authors - Daniele Simoneschi ; KO Validated
human	WB	Pharmacol Res; miR-103a-3p regulates mitophagy in Parkinson's disease through Parkin/Ambra1 signaling.; Authors - Junjun Zhou
mouse	WB	Neurobiol Dis; Neuron-astrocyte transmitophagy is altered in Alzheimer's disease.; Authors - Riikka Lampinen
human	WB,IHC	Brain Pathol; AMBRA1, a novel α-synuclein-binding protein, is implicated in the pathogenesis of multiple system atrophy.; Authors - Yasuo Miki ; KD Validated
human	WB	Front Cell Dev Biol; Autophagy-Related Genes and Long Noncoding RNAs Signatures as Predictive Biomarkers for Osteosarcoma Survival.; Authors - Jian Zhang
human	WB	Int J Biochem Cell Biol; Selenoprotein S attenuates high glucose and/or ox-LDL-induced endothelium injury by regulating Akt/mTOR signaling and autophagy.; Authors - Zinan Wang