ALK Polyklonaler Antikörper

ALK Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 24184-1-AP

Synonyme

ALK, ALK tyrosine kinase receptor, Anaplastic lymphoma kinase, CD246, TFG/ALK

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Galerie der Validierungsdaten

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  WB analysis using 24184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24184-1-AP (ALK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 24184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 24184-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human brain using 24184-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 24184-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24184-1-AP (ALK Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, MCF-7-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

24184-1-AP bindet in WB, IHC, ELISA ALK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALK fusion protein Ag21493
• Vollständiger Name: anaplastic lymphoma receptor tyrosine kinase
• Berechnetes Molekulargewicht: 1620 aa, 176 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: NM_004304
• Gene symbol: ALK
• Gene ID (NCBI): 238
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALK, also named as CD246, is a receptor tyrosine kinase (RTK) that belongs to the protein kinase superfamily. ALK is usually found in the nervous system and appears to play an important role in the normal development and function of the nervous system. ALK was originally identified as part of the NPM (Nucleophosmin)-ALK oncogenic fusion protein, resulting from the (2;5)(p23;q35) translocation that is frequently associated with anaplastic large-cell lymphoma (ALCL). The EML4 (echinoderm microtubule-associated protein-like 4)-ALK fusion protein have been described in non-small-cell lung cancer (NSCLC), this transforming fusion kinase is a promising candidate for a therapeutic target as well as for a diagnostic molecular marker in NSCLC (PMID: 17625570).

Publikationen

Species	Application	Title
human	WB	Oncol Rep; DEK promotes the proliferation and invasion of lung cancers and indicates poor prognosis in lung adenocarcinomas.; Authors - Mai-Qing Yang

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