ALDH4A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ALDH4A1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 11604-1-AP

Synonyme

ALDH4, ALDH4A1, P5C dehydrogenase, P5CD, P5CDh, P5CDhL, P5CDhS

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Galerie der Validierungsdaten

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  WB analysis of human heart using 11604-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11604-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11604-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11604-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11604-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11604-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 11604-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11604-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11604-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11604-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11604-1-AP (ALDH4A1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 11604-1-AP

  IP result of anti-ALDH4A1 (IP:11604-1-AP, 4ug; Detection:11604-1-AP 1:800) with mouse skeletal muscle tissue lysate 3600ug.

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11604-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11604-1-AP (ALDH4A1 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, HepG2-Zellen, humanes Hirngewebe, humanes Lebergewebe, humanes Skelettmuskelgewebe, K-562-Zellen, Maus-Dünndarmgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

11604-1-AP bindet in WB, IP, IHC, ELISA ALDH4A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ALDH4A1 fusion protein Ag2147
• Vollständiger Name: aldehyde dehydrogenase 4 family, member A1
• Berechnetes Molekulargewicht: 563 aa, 62 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC023600
• Gene symbol: ALDH4A1
• Gene ID (NCBI): 8659
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH4A1(Aldehyde dehydrogenase family 4 member A1) is also named as ALDH4, P5CDH,P5C dehydrogenase and belongs to the aldehyde dehydrogenase family. P5CDH is a mitochondrial matrix NAD(+)-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate.Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2).

Publikationen

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC.; Authors - Liu Wei W ; KD Validated
human	WB	Sci Rep; Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.; Authors - Bobby Jones
human,mouse	WB,IHC	J Exp Clin Cancer Res; Pharmacological targeting PTK6 inhibits the JAK2/STAT3 sustained stemness and reverses chemoresistance of colorectal cancer.; Authors - Chaoqun Liu
mouse	WB	Nat Metab; Inhibition of the proline metabolism rate-limiting enzyme P5CS allows proliferation of glutamine-restricted cancer cells; Authors - Samantha J Linder ; KD Validated