ALDH1A2 Polyklonaler Antikörper

ALDH1A2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13951-1-AP

Synonyme

ALDH1A2, RALDH 2, RALDH(II), RALDH2, RALDH2 T, Retinal dehydrogenase 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBK-562-Zellen, Maushodengewebe
Erfolgreiche IPMaushodengewebe
Erfolgreiche Detektion in IHChumanes Hodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 8 publications below
IHCSee 5 publications below

Produktinformation

13951-1-AP bindet in WB, IP, IHC, ELISA ALDH1A2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ALDH1A2 fusion protein Ag5062
Vollständiger Name aldehyde dehydrogenase 1 family, member A2
Berechnetes Molekulargewicht 518 aa, 57 kDa
Beobachtetes Molekulargewicht 53-57 kDa
GenBank-ZugangsnummerBC030589
Gene symbol ALDH1A2
Gene ID (NCBI) 8854
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ALDH1A2(aldehyde dehydrogenase family 1 member A2) is also named as RALDH2(retinal dehydrogenase 2) and belongs to the aldehyde dehydrogenase family. It is a cytosolic homotetramer(56.7 kDa subunits) exhibiting complex expression patterns throughout embryonic development. ALDH1A2 plays a role in retinoid metabolism during embryonic development where it is considered to be the major retinoic acid-synthesizing enzyme during early embryogenesis(PMID:20735668). Its expression is reduced in primary prostate tumors compared with normal prostate tissue suggested that it is normally expressed in the epithelial compartment of prostate tissue(PMID:16166285).It has 2 isoforms produced by alternative splicing.

Protokolle

Produktspezifische Protokolle
WB protocol for ALDH1A2 antibody 13951-1-APProtokoll herunterladen
IHC protocol for ALDH1A2 antibody 13951-1-APProtokoll herunterladen
IP protocol for ALDH1A2 antibody 13951-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
humanWB

Elife

FAK activity sustains intrinsic and acquired ovarian cancer resistance to platinum chemotherapy.

Authors - Carlos J Diaz Osterman
humanWB

iScience

Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression

Authors - Zhilei Zhang
mouseWB

J Nutr Biochem

All-trans retinoic acid induces lipophagy through the activation of the AMPK-Beclin1 signaling pathway and reduces Rubicon expression in adipocytes

Authors - Yuki Mori
humanWB

Am J Cancer Res

Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression

Authors - Bing-Yen Wang
humanIHC

J Lipid Res

Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations.

Authors - Samuel L Arnold