AK3 Polyklonaler Antikörper

AK3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12562-1-AP

Synonyme

AKL3L1, AKL3L, AK6, AK3L1, AK 3

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Galerie der Validierungsdaten

• 

  WB analysis of human placenta using 12562-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12562-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12562-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12562-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12562-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12562-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12562-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12562-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12562-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12562-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12562-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12562-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12562-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12562-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12562-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12562-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12562-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12562-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12562-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12562-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12562-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12562-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12562-1-AP (AK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12562-1-AP

  IP result of anti-AK3 (IP:12562-1-AP, 3ug; Detection:12562-1-AP 1:500) with mouse kidney tissue lysate 4400ug.

• 

  IHC staining of human pancreas cancer using 12562-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12562-1-AP (AK3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12562-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12562-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12562-1-AP (AK3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 12562-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12562-1-AP (AK3 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe, BxPC-3-Zellen, HEK-293-Zellen, HepG2-Zellen, humanes Nierengewebe, L02-Zellen, Mausherzgewebe, Mausnierengewebe, MCF-7-Zellen, Y79-Zellen
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12562-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA AK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AK3 fusion protein Ag3146
• Vollständiger Name: adenylate kinase 3
• Berechnetes Molekulargewicht: 227 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC013771
• Gene symbol: AK3
• Gene ID (NCBI): 50808
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AK3(Adenylate kinase 3) is also named as AK3L1, AK6, AKL3L and belongs to the adenylate kinase family. It is located in the mitochondrial matrix and is a GTP:AMP phosphotransferase(PMID:2546792). AK3 can be detected AK3 at an apparent molecular mass of 28 kD by western blot analysis and subcellular fractionation of mouse liver and kidney detected Ak3 in the mitochondrial fraction(PMID:11485571). This antibody may also recognize AK4 due to the high homology.

Publikationen

Species	Application	Title
human	WB	Mol Med Rep; MicroRNA‑214 upregulates HIF‑1α and VEGF by targeting ING4 in lung cancer cells.; Authors - Yue Li
human	WB	J Gene Med; MiR-96-5p promotes breast cancer migration by activating MEK/ERK signaling.; Authors - Wei-Yan Qin