AK2 Polyklonaler Antikörper

AK2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 11014-1-AP

Synonyme

ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, ADK2, Adenylate monophosphate kinase, Adenylate kinase 2, mitochondrial, N-terminally processed

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Galerie der Validierungsdaten

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  WB analysis using 11014-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11014-1-AP (AK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11014-1-AP

  IP result of anti-AK2 (IP:11014-1-AP, 3ug; Detection:11014-1-AP 1:800) with mouse liver tissue lysate 12500ug.

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  IHC staining of human pancreas cancer using 11014-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11014-1-AP (AK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11014-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11014-1-AP (AK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11014-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AK2 antibody (11014-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, Mausnierengewebe, Rattenlebergewebe, Rattennierengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

11014-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA AK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AK2 fusion protein Ag1464
• Vollständiger Name: adenylate kinase 2
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC009405
• Gene symbol: AK2
• Gene ID (NCBI): 204
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
zebrafish	IHC	Nat Genet; Reticular dysgenesis (aleukocytosis) is caused by mutations in the gene encoding mitochondrial adenylate kinase 2.; Authors - Pannicke Ulrich U
human	WB,IF	Blood; The palmitoyltransferase ZDHHC21 regulates oxidative phosphorylation to induce differentiation block and stemness in AML; Authors - Xuejing Shao
human	WB	Neuron; Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.; Authors - Nathalie Escande-Beillard
human	WB	J Exp Med; A loss-of-function NUAK2 mutation in humans causes anencephaly due to impaired Hippo-YAP signaling.; Authors - Carine Bonnard
human	WB	EMBO Mol Med; A progeroid syndrome caused by a deep intronic variant in TAPT1 is revealed by RNA/SI-NET sequencing; Authors - Nasrinsadat Nabavizadeh
human	WB,IF	Fluids Barriers CNS; Cocaine regulates antiretroviral therapy CNS access through pregnane-x receptor-mediated drug transporter and metabolizing enzyme modulation at the blood brain barrier; Authors - Rodnie Colón Ortiz