AGR3 Polyklonaler Antikörper

AGR3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11967-1-AP

Synonyme

hAG 3, Anterior gradient protein 3, Anterior gradient 3 homolog, AG-3, AG3

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Galerie der Validierungsdaten

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  WB analysis of SKOV-3 using 11967-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 11967-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11967-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11967-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11967-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11967-1-AP (AGR3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 11967-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11967-1-AP (AGR3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SKOV-3 using 11967-1-AP

  Immunofluorescent analysis of SKOV-3 cells using 11967-1-AP (AGR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SKOV-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Produktinformation

11967-1-AP bindet in WB, IHC, IF/ICC, ELISA AGR3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Huhn
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AGR3 fusion protein Ag2577
• Vollständiger Name: anterior gradient homolog 3 (Xenopus laevis)
• Berechnetes Molekulargewicht: 166 aa, 19 kDa
• Beobachtetes Molekulargewicht: 19-25 kDa
• GenBank-Zugangsnummer: BC058284
• Gene symbol: AGR3
• Gene ID (NCBI): 155465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
chicken	IF	Elife; Single-cell proteomics reveals changes in expression during hair-cell development.; Authors - Ying Zhu
human	WB,IHC	Oncotarget; Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.; Authors - Soma Samanta
human	IHC, IF, WB	Front Pharmacol; AGR3 Regulates Airway Epithelial Junctions in Patients with Frequent Exacerbations of COPD.; Authors - Rui Ye
chicken	IF	iScience; Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium; Authors - Mami Matsunaga
human	IF	J Transl Med; Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers; Authors - Youxue Zhang