ADRP/Perilipin-2 Monoklonaler Antikörper

ADRP/Perilipin-2 Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G5D4

Kat-Nr. : 60340-1-Ig

Synonyme

ADFP, Adipophilin, ADRP, ADRP/Perilipin 2, Perilipin 2, Perilipin-2, PLIN2

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Galerie der Validierungsdaten

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  WB analysis of human adipose using 60340-1-Ig

  human adipose tissue were subjected to SDS PAGE followed by western blot with 60340-1-Ig (ADRP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brown adipose using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 60340-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Fettgewebe
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, braunes Mausfettgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

60340-1-Ig bindet in WB, IHC, ELISA ADRP/Perilipin-2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ADRP/Perilipin-2 fusion protein Ag7643
• Vollständiger Name: adipose differentiation-related protein
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 47-52 kDa
• GenBank-Zugangsnummer: BC005127
• Gene symbol: ADFP
• Gene ID (NCBI): 123
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.