Adrenomedullin Polyklonaler Antikörper

Adrenomedullin Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 10778-1-AP

Synonyme

ADM, ProAM N terminal 20 peptide, Proadrenomedullin N-20 terminal peptide, Pro-adrenomedullin, PAMP

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Galerie der Validierungsdaten

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  WB analysis of human kidney using 10778-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 10778-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 10778-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10778-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10778-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10778-1-AP (Adrenomedullin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10778-1-AP (Adrenomedullin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10778-1-AP (Adrenomedullin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10778-1-AP (Adrenomedullin antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 10778-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, A549-Zellen, fetales humanes Hirngewebe, humanes Plazenta-Gewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Nierengewebe, humanes Pankreaskarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

10778-1-AP bindet in WB, IHC, IF/ICC, ELISA Adrenomedullin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Adrenomedullin fusion protein Ag1197
• Vollständiger Name: adrenomedullin
• Berechnetes Molekulargewicht: 20 kDa
• Beobachtetes Molekulargewicht: 6 kDa
• GenBank-Zugangsnummer: BC015961
• Gene symbol: Adrenomedullin/ADM
• Gene ID (NCBI): 133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) are two small active hormones derived from the expression of a single gene (Adm) that is expressed throughout the GI tract, including the mucosal epithelium, glandular duct cells, neuroendocrine cells, and smooth muscle cells of the GI tract, between the oral cavity and the rectum (PMID:10782362, PMID:27345325). These two peptides coexist in GI cells, where they regulate many physiological functions including vasodilation, angiogenesis, anti-inflammation, organ protection, and tissue repair. AM suppresses inflammatory cytokine production in the intestinal mucosa, improves vascular and lymphatic function, mucosal epithelial repair, and intestinal barrier function in animal models with intestinal inflammation (PMID:27965594, PMID:29311984). Molecular mass species of 18, 14, and 6 kDa were identified in tumor cell lysates and presumably represent AM precursor, processed intermediates, and the authentic peptide, respectively. There is also a 22-kDa immunoreactive species in two cancer cell lines, H720 and MCF-7 (PMID: 8798536).

Publikationen

Species	Application	Title
human	IF	Cells; Differentiation Trajectory of Limbal Stem and Progenitor Cells under Normal Homeostasis and upon Corneal Wounding.; Authors - Zhenwei Song
human	IHC	Cell Mol Life Sci; APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b.; Authors - Giulia Antoniali
human	WB	Prostate; Prostate fibroblasts enhance androgen receptor splice variant 7 expression in prostate cancer cells; Authors - Takeshi Sasaki
human	WB	CNS Neurosci Ther; Identification and validation of a glycolysis-related taxonomy for improving outcomes in glioma; Authors - Tianshu Ying