ADCY3 Polyklonaler Antikörper

ADCY3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 19492-1-AP

Synonyme

AC III, AC3, ADCY3, adenylate cyclase 3, Adenylate cyclase type 3, Adenylate cyclase type III, Adenylyl cyclase 3, ATP pyrophosphate lyase 3, KIAA0511

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 19492-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19492-1-AP (ADCY3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19492-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19492-1-AP (ADCY3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MDCK using 19492-1-AP

  IP Result of anti-ADCY3 (IP:19492-1-AP, 4ug; Detection:19492-1-AP 1:500) with MDCK cells lysate 3500ug.

• 

  IHC staining of human kidney using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19492-1-AP (ADCY3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19492-1-AP (ADCY3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 19492-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDCK using 19492-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 19492-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using ADCY3 antibody (19492-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 19492-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using ADCY3 antibody (19492-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Mouse embryonic fibroblasts using 19492-1-AP

  IF result from Dr. Corbit, Kevin. anti-ADCY3 (19492-1-AP) mark the cilium of Mouse embryonic fibroblasts.

• 

  IF Staining of MDCK using 19492-1-AP

  Immunofluorescent analysis of MDCK cells using 19492-1-AP (ADCY3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Hirngewebe
• Erfolgreiche IP: MDCK-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MDCK-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 7 publications below

Produktinformation

19492-1-AP bindet in WB, IP, IHC, IF, ELISA ADCY3 und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: adenylate cyclase 3
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 170-180 kDa
• GenBank-Zugangsnummer: NM_004036
• Gene symbol: ADCY3
• Gene ID (NCBI): 109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADCY3 is an adenylate cyclase (AC) functioning to convert ATP to cAMP in response to signals initiated by activation of Gs-coupled receptors. To date, 10 isoforms of AC have been cloned in mammals. Initially identified in olfactory cilia, ADCY3 is regarded as the olfactory isoform and required for detection of odorants. Recently it has been found that outside of the olfactory epithelium the olfactory-like signaling pathway including AC3 may also have a key role in spermatogenesis and spermatozoa functions. In addition, the ciliary location of AC3 makes it as a prominent marker for primary cilia in brain.AC3 is typically found at 130 kDa in olfactory tissues.In nonolfactory tissues (such as sperm), this protein is reported to migrate with a molecular mass of 55 kDa,occasionally accompanied by additional bands at either 90 or 130 kDa(PMID:9539154).

Publikationen

Species	Application	Title
mouse	IHC	Acta Physiol (Oxf); Aromatherapy: Activating Olfactory Calcium-sensing Receptors Impairs Renal Hemodynamics via Sympathetic Nerve-mediated Vasoconstriction.; Authors - Shih-Pin Lee
mouse	WB,IF	Hum Mol Genet; Shortened primary cilium length and dysregulated Sonic hedgehog signaling in Niemann-Pick C1 disease.; Authors - Sonia Canterini
rat	IF	Biomolecules; Preferential Expression of Ca2+-Stimulable Adenylyl Cyclase III in the Supraventricular Area, including Arrhythmogenic Pulmonary Vein of the Rat Heart.; Authors - Yosuke Okamoto
	WB,IHC	Front Physiol; Expression Regulation of Water Reabsorption Genes and Transcription Factors in the Kidneys of Lepus yarkandensis.; Authors - Shengjie Luo
mouse	IF	Dev Dyn; Phosphosite T674A mutation in kinesin family member 3A fails to reproduce tissue and ciliary defects characteristic of CILK1 loss of function.; Authors - Casey D Gailey
human	IF	Phytochemistry; Hypoglycemic flavonoids from Selaginella tamariscina (P.Beauv.) Spring.; Authors - Hong-Ping Long