ADAT2 Polyklonaler Antikörper

ADAT2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13621-1-AP

Synonyme

tRNA-specific adenosine deaminase 2, TAD2, EC:3.5.4.33, Deaminase domain-containing protein 1, DEADC1

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Galerie der Validierungsdaten

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  WB analysis of A431 using 13621-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13621-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 13621-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 13621-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 13621-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13621-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13621-1-AP (ADAT2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13621-1-AP

  IP result of anti-ADAT2 (IP:13621-1-AP, 4ug; Detection:13621-1-AP 1:300) with mouse brain tissue lysate 3000 ug.

• 

  IHC staining of human hepatocirrhosis using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13621-1-AP (ADAT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 13621-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 13621-1-AP

  Immunofluorescent analysis of A431 cells using 13621-1-AP (ADAT2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, Maushirngewebe, Maus-Pankreasgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

13621-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ADAT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAT2 fusion protein Ag4529
• Vollständiger Name: adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 191 aa, 21 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC037955
• Gene symbol: ADAT2
• Gene ID (NCBI): 134637
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAT2(tRNA-specific adenosine deaminase 2) is also named as DEADC1 and Belongs to the cytidine and deoxycytidylate deaminase family. It is a 24-kDa molecular mass protein that harbors all the conserved motifs required for deamination. ADAT2 and ADAT3 can function as a heterodimer which catalyses inosine formation at the wobble position (position 34) in eukaryotic tRNAs(PMID:12457566). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Nucleic Acids Res; The structure of the mouse ADAT2/ADAT3 complex reveals the molecular basis for mammalian tRNA wobble adenosine-to-inosine deamination.; Authors - Elizabeth Ramos-Morales