ADAM12 Polyklonaler Antikörper

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• KD/KO Validated

ADAM12 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

39

Kat-Nr. : 14139-1-AP

Synonyme

ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 14139-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14139-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14139-1-AP (ADAM12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14139-1-AP

  IP Result of anti-ADAM12 (IP:14139-1-AP, 4ug; Detection:14139-1-AP 1:300) with HeLa cells lysate 4650ug.

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  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14139-1-AP (ADAM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 14139-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 14139-1-AP (ADAM12 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 14139-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ADAM12 antibody (14139-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 14139-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ADAM12 (14139-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Mausherzgewebe, MCF-7-Zellen, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 24 publications below
• IHC: See 15 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Produktinformation

14139-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ADAM12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADAM12 fusion protein Ag5206
• Vollständiger Name: ADAM metallopeptidase domain 12
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa, 70-80 kDa
• GenBank-Zugangsnummer: BC060804
• Gene symbol: ADAM12
• Gene ID (NCBI): 8038
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors(PMID:17355265). ADAM12's ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein's role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kDa full-length form, 90kd mature (processed form that lack the prodomain), 50-68kDa degradation product and 27kDa prodomain.

Publikationen

Species	Application	Title
mouse,human	IF	Cell Stem Cell; Vaccination-based immunotherapy to target profibrotic cells in liver and lung; Authors - Michal Sobecki
human	IHC	J Cell Biol; Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.; Authors - Díaz Bego?a B
human	IF	Theranostics; Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.; Authors - Yinan Chen
human	WB,IF	Cell Death Differ; ADAM12-directed ectodomain shedding of E-cadherin potentiates trophoblast fusion.; Authors - M Aghababaei
human	IHC	J Hematol Oncol; Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway.; Authors - Kosuke Ogawa
human	WB,IHC	Oncogene; A positive feedback loop between HER2 and ADAM12 in human head and neck cancer cells increases migration and invasion.; Authors - Rao V H VH

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