ADA Polyklonaler Antikörper

ADA Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (2)

Kat-Nr. : 13328-1-AP

Synonyme

EC:3.5.4.4, ADA1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 13328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 13328-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13328-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 13328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse small intestine using 13328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ADA antibody (13328-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Sp2/0-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Tonsillitisgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 2 publications below

Produktinformation

13328-1-AP bindet in WB, IHC, IF-P, ELISA ADA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ADA fusion protein Ag4128
• Vollständiger Name: adenosine deaminase
• Berechnetes Molekulargewicht: 363 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45-47 kDa
• GenBank-Zugangsnummer: BC040226
• Gene symbol: ADA
• Gene ID (NCBI): 100
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB, IHC	Front Cell Dev Biol; Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production.; Authors - Xiaoting Chen
mouse	WB	Eur J Pharmacol; Adenosine kinase (ADK) inhibition with ABT-702 induces ADK protein degradation and a distinct form of sustained cardioprotection.; Authors - Gerald Wölkart
mouse	WB,IHC	Cell Death Dis; Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation; Authors - Mei-Xin Li
human	WB	bioRxiv; The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma; Authors - Ying-Jiun C Chen
	WB	NPJ Biofilms Microbiomes; Lactobacillus rhamnosus GG ameliorates hyperuricemia in a novel model; Authors - Yang Fu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Neta (Verified Customer) (07-28-2022)

antibody worked, signal slightly different than reported but overall OK.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse small intestine

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with NaCit antigen retrieval ph=6.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (neurons) at 3dpf

ADA Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (neurons) at 3dpf (Cat no:13328-1-AP)