ACVRL1 Polyklonaler Antikörper

ACVRL1 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 14745-1-AP

Synonyme

Activin receptor like kinase 1, Activin receptor-like kinase 1, ACVRLK1, ALK 1, ALK1

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  WB analysis of human placenta using 14745-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14745-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14745-1-AP (ACVRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14745-1-AP

  IP result of anti-ACVRL1 (IP:14745-1-AP, 4ug; Detection:14745-1-AP 1:300) with Jurkat cells lysate 2400ug.

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  IHC staining of human heart using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14745-1-AP (ACVRL1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lymphoma using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 14745-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14745-1-AP (ACVRL1 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Jurkat using 14745-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug ACVRL1 Polyclonal antibody (14745-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plazenta-Gewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14745-1-AP bindet in WB, IHC, IF, FC (Intra), IP, ELISA ACVRL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACVRL1 fusion protein Ag6449
• Vollständiger Name: activin A receptor type II-like 1
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC042637
• Gene symbol: ACVRL1
• Gene ID (NCBI): 94
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

Publikationen

Species	Application	Title
mouse,human	WB	Mol Cell; OTULIN allies with LUBAC to govern angiogenesis by editing ALK1 linear polyubiquitin.; Authors - Yesheng Fu
human	WB	Nat Commun; Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils.; Authors - Yusuke Kimishima
human	WB	J Mol Cell Cardiol; BMP9 attenuates occurrence of venous malformation by maintaining endothelial quiescence and strengthening vessel walls via SMAD1/5/ID1/α-SMA pathway.; Authors - Yongyun Li
human	WB,IP	Cell Biol Int; CHRDL2 promotes osteosarcoma cell proliferation and metastasis through the BMP-9/ PI3K/ AKT pathway.; Authors - Houping Chen
human	IHC	J Thorac Dis; Pulmonary inflammatory myofibroblastic tumor versus IgG4-related inflammatory pseudotumor: differential diagnosis based on a case series.; Authors - Longfei Zhu
mouse	WB,IHC	Biosci Biotechnol Biochem; BMP9 functions as a negative regulator in the myogenic differentiation of primary mouse myoblasts; Authors - Xiya Lu