ACTN2 Monoklonaler Antikörper

ACTN2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1D5F9

Kat-Nr. : 68223-1-Ig

Synonyme

actinin, alpha 2, ACTN2, Alpha actinin 2, CMD1AA, F actin cross linking protein

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Galerie der Validierungsdaten

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  WB analysis using 68223-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68223-1-Ig (ACTN2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 68223-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68223-1-Ig (ACTN2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Herzgewebe, Hausschwein-Kolongewebe, Kaninchenherzgewebe, Mausherzgewebe, Rattenherzgewebe, Ratten-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68223-1-Ig bindet in WB, ELISA ACTN2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ACTN2 fusion protein Ag5578
• Vollständiger Name: actinin, alpha 2
• Berechnetes Molekulargewicht: 104 kDa
• Beobachtetes Molekulargewicht: 103 kDa
• GenBank-Zugangsnummer: BC051770
• Gene symbol: ACTN2
• Gene ID (NCBI): 88
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha actinin 2 (ACTN2) belongs to the alpha-actinin family and is expressed in both skeletal and cardiac muscles and functions to anchor myofibrillar actin thin filaments and titin to Z-discs (PMID: 30701273). ACTN2 is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Mutations in ACTN2 are associated with hypertrophic cardiomyopathy, as well as dilated cardiomyopathy and endocardial fibroelastosis (PMID: 20022194, 14567970).