ACSS1 Monoklonaler Antikörper

ACSS1 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3E10F11

Kat-Nr. : 68608-1-Ig

Synonyme

ACAS2L, AceCS2, AceCS2L, Acetate CoA ligase 2, Acetyl CoA synthetase 2, ACSS1, FLJ45659, KIAA1846



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Plazenta-Gewebe, Caco-2-Zellen, HepG2-Zellen, humanes Herzgewebe, Mausherzgewebe, Rattenherzgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

68608-1-Ig bindet in WB, ELISA ACSS1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen ACSS1 fusion protein Ag10853
Vollständiger Name acyl-CoA synthetase short-chain family member 1
Berechnetes Molekulargewicht 689 aa, 75 kDa
Beobachtetes Molekulargewicht 70-75 kDa
GenBank-ZugangsnummerBC039261
Gene symbol ACSS1
Gene ID (NCBI) 84532
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.

Protokolle

Produktspezifische Protokolle
WB protocol for ACSS1 antibody 68608-1-IgProtokoll herunterladen
Standard-Protokolle
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