ACSL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ACSL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen

44

Rezensionen (1)

Kat-Nr. : 13989-1-AP

Synonyme

EC:6.2.1.24, EC:6.2.1.15, Arachidonate--CoA ligase, Acyl-CoA synthetase 1, Acyl CoA synthetase 1

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Galerie der Validierungsdaten

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  WB analysis using 13989-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13989-1-AP (ACSL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 13989-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 13989-1-AP (ACSL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 13989-1-AP

  IP result of anti-ACSL1 (IP:13989-1-AP, 4ug; Detection:13989-1-AP 1:1000) with mouse liver tissue lysate 3040 ug.

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  IHC staining of human lung cancer using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13989-1-AP (ACSL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13989-1-AP (ACSL1 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human heart using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13989-1-AP (ACSL1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 13989-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13989-1-AP (ACSL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 13989-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13989-1-AP (ACSL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Maus-Cerebellum-Gewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Herzgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 40 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

13989-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA, Cell treatment ACSL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ACSL1 fusion protein Ag5059
• Vollständiger Name: acyl-CoA synthetase long-chain family member 1
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 68 kDa, 78 kDa
• GenBank-Zugangsnummer: BC050073
• Gene symbol: ACSL1
• Gene ID (NCBI): 2180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACSL1(Long-chain-fatty-acid--CoA ligase 1) is also named as FACL1, FACL2, LACS, LACS1, LACS2 and belongs to the ATP-dependent AMP-binding enzyme family. ACSL1 is a 75 kDa protein that is associated peripherally with the plasma membrane(Brian M Wiczer, etc., 2006). ACSL1 is abundantly expressed in tissues, such as liver and brown fat, that metabolize substantial amounts of triglycerides as fuel, and as such, a deficiency in ACSL1 function could have a more profound affect in those cells, resulting in hepatosteatosis and potentially increased very low density lipoprotein production by the liver or decreased thermogenic capacity in brown adipose tissue(PMID:19429676). An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages (PMID:17259370). It has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
human	WB	Hepatology; Acetyl-CoA carboxylase α promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.; Authors - Ming-Da Wang
human	IF	Theranostics; Mesenchymal glioblastoma constitutes a major ceRNA signature in the TGF-β pathway.; Authors - Qixue Wang
human	WB, IHC	Cancer Res; Long noncoding RNA HULC modulates abnormal lipid metabolism in hepatoma cells through an miR-9-mediated RXRA signaling pathway.; Authors - Ming Cui
mouse	WB	Oxid Med Cell Longev; Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.; Authors - Wei Wang
human	WB	Cell Rep; HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression.; Authors - De Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Thomas (Verified Customer) (10-23-2019)

Very good service, very good antibody

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: Rattus heart