Aconitase 1 Polyklonaler Antikörper

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• KD/KO Validated

Aconitase 1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 12406-1-AP

Synonyme

ACO1, ACO 1, EC:4.2.1.3, Citrate hydro-lyase, Aconitase1

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Galerie der Validierungsdaten

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  WB analysis using 12406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12406-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12406-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12406-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12406-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12406-1-AP (Aconitase 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 12406-1-AP

  IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:5000) with HepG2 cells lysate 1925 ug.

• 

  IP experiment of mouse liver using 12406-1-AP

  IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:300) with mouse liver tissue lysate 2400 ug.

• 

  IP experiment of HepG2 using 12406-1-AP

  IP result of anti-Aconitase 1 (IP:12406-1-AP, 4ug; Detection:12406-1-AP 1:5000) with HepG2 cells lysate 1925 ug.

• 

  IF Staining of U2OS using 12406-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 12406-1-AP (Aconitase 1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Mauslebergewebe, Mausnierengewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Produktinformation

12406-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Aconitase 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Aconitase 1 fusion protein Ag3083
• Vollständiger Name: aconitase 1, soluble
• Berechnetes Molekulargewicht: 889 aa, 98 kDa
• Beobachtetes Molekulargewicht: 98 kDa
• GenBank-Zugangsnummer: BC018103
• Gene symbol: Aconitase 1
• Gene ID (NCBI): 48
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian cells possess two distinct isoforms of aconitase encoded by different genes: Aconitase 1 [ACO1; cytosolic aconitase (c-aconitase)] encoded by ACO1 and Aconitase 2 [ACO2; mitochondrial aconitase (m-aconitase)] encoded by ACO2. In addition to the enzymatic roles in the cytoplasm, ACO1 also is known to bind to mRNAs of specific genes and maintain iron homeostasis.

Publikationen

Species	Application	Title
human	WB	iScience; Asparagine starvation suppresses histone demethylation through iron depletion; Authors - Jie Jiang ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Iron deposition-induced ferroptosis in alveolar type II cells promotes the development of pulmonary fibrosis.; Authors - Haipeng Cheng
human,mouse	WB,IHC	Cell Biosci; Mutant huntingtin induces iron overload via up-regulating IRP1 in Huntington's disease.; Authors - Li Niu
human	WB	Drug Des Devel Ther; Curcumin induces apoptosis and protective autophagy in castration-resistant prostate cancer cells through iron chelation.; Authors - Chunguang Yang
human	WB	Biomolecules; Carbamoyl-Phosphate Synthase 1 as a Novel Target of Phomoxanthone A, a Bioactive Fungal Metabolite.; Authors - Sara Ceccacci
human	WB	JOJ Ophthalmol; Mitophagy, Ferritinophagy and Ferroptosis in Retinal Pigment Epithelial Cells Under High Glucose Conditions: Implications for Diabetic Retinopathy and Age-Related Retinal Diseases.; Authors - Lalit Pukhrambam Singh