PHACS Polyklonaler Antikörper

PHACS Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11491-1-AP

Synonyme

ACC synthase like protein 1, ACCS, ACS, PHACS

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Galerie der Validierungsdaten

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  WB analysis of MDA-MB-453s using 11491-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11491-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11491-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11491-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11491-1-AP (PHACS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11491-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11491-1-AP (PHACS antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen, HeLa-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11491-1-AP bindet in WB, IHC, ELISA PHACS und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PHACS fusion protein Ag2056
• Vollständiger Name: 1-aminocyclopropane-1-carboxylate synthase homolog (Arabidopsis)(non-functional)
• Berechnetes Molekulargewicht: 501 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC020197
• Gene symbol: PHACS
• Gene ID (NCBI): 84680
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ACCS(1-aminocyclopropane-1-carboxylate synthase-like protein 1) is also named as PHACS and belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. The deduced 501-amino acid protein has a calculated molecular mass of 58 kD. It contains overlapping aminotransferase I and beta-eliminating lyase domains, and it shares structural similarity with aspartate aminotransferase, tyrosine aminotransferase, and enzymes that catalyze beta-elimination reactions on amino acids.