ABHD5 Polyklonaler Antikörper

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• KD/KO Validated

ABHD5 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

26

Kat-Nr. : 12201-1-AP

Synonyme

IECN2, EC:2.3.1.51, CGI58, CDS, Abhydrolase domain-containing protein 5

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Galerie der Validierungsdaten

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  WB analysis using 12201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12201-1-AP (ABHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12201-1-AP

  IP result of anti-ABHD5 (IP:12201-1-AP, 4ug; Detection:12201-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

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  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

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  IF Staining of MCF-7 using 12201-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ABHD5 antibody 12201-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A549-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, LNCaP-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

12201-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA ABHD5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABHD5 fusion protein Ag2840
• Vollständiger Name: abhydrolase domain containing 5
• Berechnetes Molekulargewicht: 349 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC021958
• Gene symbol: ABHD5
• Gene ID (NCBI): 51099
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.; Authors - Yunfan Yang
mouse	WB,CoIP	Cell Death Differ; Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation.; Authors - Yi Wu
human	WB	Proc Natl Acad Sci U S A; Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover.; Authors - Olzmann James A JA
mouse	WB,IP,IF	Proc Natl Acad Sci U S A; Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.; Authors - Satish Patel
human	WB	J Cell Biol; ORP5 localizes to ER-lipid droplet contacts and regulates the level of PI(4)P on lipid droplets.; Authors - Ximing Du
human	WB	Elife; Inhibition of intracellular lipolysis promotes human cancer cell adaptation to hypoxia.; Authors - Xiaodong Zhang