ABCG1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ABCG1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA, IF

Konjugation

Unkonjugiert

Publikationen

44

Rezensionen (1)

Kat-Nr. : 13578-1-AP

Synonyme

ABC8, ABCG1, White protein homolog, WHITE1, WHT1

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Galerie der Validierungsdaten

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  WB analysis of RAW 264.7 using 13578-1-AP

  WB result of ABCG1 antibody (13578-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABCG1 transfected RAW 264.7 cells.

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  WB analysis using 13578-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13578-1-AP (ABCG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13578-1-AP

  IP result of anti-ABCG1 (IP:13578-1-AP, 4ug; Detection:13578-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse placenta using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 13578-1-AP (ABCG1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 13578-1-AP (ABCG1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP (ABCG1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13578-1-AP (ABCG1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human tonsillitis using 13578-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13578-1-AP (ABCG1 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, HepG2-Zellen, Mausnierengewebe, Rattennierengewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maus-Plazenta-Gewebe, humanes Tonsillitisgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 40 publications below
• IHC: See 6 publications below
• IF: See 6 publications below

Produktinformation

13578-1-AP bindet in WB, IHC, IP, ELISA, IF ABCG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABCG1 fusion protein Ag4479
• Vollständiger Name: ATP-binding cassette, sub-family G (WHITE), member 1
• Berechnetes Molekulargewicht: 678 aa, 76 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC029158
• Gene symbol: ABCG1
• Gene ID (NCBI): 9619
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCG1 is a cholesterol and phospholipid transporter expressed in lung, brain, spleen, and macrophages. In liver, ABCG1 is expressed mainly in Kupffer cells. The monomer of ABCG1 is around 60-65 kDa, while multiple forms of ABCG1 can be observed on SDS-PAGE, including 130 kDa (dimer), 90-95 kDa, 110 kDa. It was not clear whether the glycosylation or splicing accounts for the differences in mass. (PMID: 16702602)

Publikationen

Species	Application	Title
human	WB	Cancer Res; PRMT1 sustains de novo fatty acid synthesis by methylating PHGDH to drive chemoresistance in triple-negative breast cancer; Authors - Takehiro Yamamoto
mouse	WB	Autophagy; Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells.; Authors - Jun Matsuda
human	WB	Br J Pharmacol; Functional interplay between LXR and AMPKα inhibits atherosclerosis in apoE deficient mice - A new anti-atherogenic strategy.; Authors - Chuanrui Ma
mouse	WB,IHC	J Control Release; Artemisinin and Procyanidins loaded multifunctional nanocomplexes alleviate atherosclerosis via simultaneously modulating lipid influx and cholesterol efflux.; Authors - Hongyan Zhou
mouse	WB	Br J Pharmacol; Targeting macrophage liver X receptors by hydrogel-encapsulated T0901317 reduces atherosclerosis without effect on hepatic lipogenesis.; Authors - Chuanrui Ma
mouse	IF	Carbohydr Polym; Cyclodextrin boostered-high density lipoprotein for antiatherosclerosis by regulating cholesterol efflux and efferocytosis.; Authors - Yanyan Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shiford (Verified Customer) (09-24-2023)

We sorted cancer stem cell subpopulations from multiple cell lines by flow cytometry and used a cluster of antibodies related to stem cell characteristics to detect. ABCG1 is one of the possible stem cell markers, with clear bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1：1500
• Cell Tissue Type: Cancer cell lines from various tissues

ABCG1 Antibody Western Blot validation (1：1500 dilution) in Cancer cell lines from various tissues (Cat no:13578-1-AP)

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