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ABCE1 Monoklonaler Antikörper

ABCE1 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1B7E8

Kat-Nr. : CL594-67960

Synonyme

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated ABCE1 Monoclonal antibody (CL594-67960)

    IF Staining of HeLa using CL594-67960

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 ABCE1 antibody (CL594-67960, Clone: 1B7E8 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®594-conjugated ABCE1 Monoclonal antibody (CL594-67960)

    FC experiment of HeLa using CL594-67960

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ABCE1 (CL594-67960, Clone:1B7E8) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen
Erfolgreiche Detektion in FCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67960 bindet in IF, FC (Intra) ABCE1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen ABCE1 fusion protein Ag28493
Vollständiger Name ATP-binding cassette, sub-family E (OABP), member 1
Berechnetes Molekulargewicht 599 aa, 67 kDa
Beobachtetes Molekulargewicht68 kDa
GenBank-ZugangsnummerBC016283
Gene symbol ABCE1
Gene ID (NCBI) 6059
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCE1 is an ATPase that is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily, it is an essential and highly conserved protein that is required for both eukaryotic translation initiation as well as ribosome biogenesis.

Protokolle

Produktspezifische Protokolle
IF protocol for CL594 ABCE1 antibody CL594-67960Protokoll herunterladen
FC protocol for CL594 ABCE1 antibody CL594-67960Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen