ABCD1 Polyklonaler Antikörper

ABCD1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11159-1-AP

Synonyme

EC:3.1.2.-, ATP-binding cassette sub-family D member 1, ATP binding cassette sub family D member 1, ALD, ABCD 1

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Galerie der Validierungsdaten

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  WB analysis of human brain using 11159-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 11159-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11159-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11159-1-AP (ABCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11159-1-AP

  IP result of anti-ABCD1 (IP:11159-1-AP, 4ug; Detection:11159-1-AP 1:500) with mouse liver tissue lysate 5000ug.

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  IHC staining of human lung cancer using 11159-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11159-1-AP

  Immunofluorescent analysis of HeLa cells using 11159-1-AP (ABCD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HeLa-Zellen, Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

11159-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA ABCD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ABCD1 fusion protein Ag1635
• Vollständiger Name: ATP-binding cassette, sub-family D (ALD), member 1
• Berechnetes Molekulargewicht: 745 aa, 83 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC025358
• Gene symbol: ABCD1
• Gene ID (NCBI): 215
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); Prostate Cancer Proliferation Is Affected by the Subcellular Localization of MCT2 and Accompanied by Significant Peroxisomal Alterations.; Authors - Isabel Valença
mouse	IF	J Biomed Res; Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis; Authors - Yejin Yao