P glycoprotein Polyklonaler Antikörper

P glycoprotein Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

72

Rezensionen (2)

Kat-Nr. : 22336-1-AP

Synonyme

ABC20, ABCB1, CD243, CLCS, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein, P glycoprotein 1, P-gp

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Galerie der Validierungsdaten

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  WB analysis using 22336-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22336-1-AP (P glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 22336-1-AP

  IP Result of anti-P glycoprotein (IP:22336-1-AP, 4ug; Detection:22336-1-AP 1:300) with human placenta tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP (P glycoprotein antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP (P glycoprotein antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22336-1-AP (P glycoprotein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: ungekochte HeLa-Zellen, ungekochte HepG2-Zellen
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 66 publications below
• IHC: See 12 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

22336-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA P glycoprotein und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: P glycoprotein fusion protein Ag17811
• Vollständiger Name: ATP-binding cassette, sub-family B (MDR/TAP), member 1
• Berechnetes Molekulargewicht: 1280 aa, 142 kDa
• Beobachtetes Molekulargewicht: 130-150 kDa
• GenBank-Zugangsnummer: BC130424
• Gene symbol: ABCB1
• Gene ID (NCBI): 5243
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.

Publikationen

Species	Application	Title
human	WB	Drug Resist Updat; Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer; Authors - Wei Zhang
human	WB,IHC	Mol Cancer; Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.; Authors - Jianyou Gu
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	J Control Release; cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancer; Authors - Xiaomei Liang
human	WB	ACS Appl Mater Interfaces; TPGS-Galactose-Modified Polydopamine Co-delivery Nanoparticles of Nitric Oxide Donor and Doxorubicin for Targeted Chemo-Photothermal Therapy against Drug-Resistant Hepatocellular Carcinoma.; Authors - Zijing Du
human	WB	ACS Appl Mater Interfaces; PMID: 31718159; Authors - Tengfei Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SABRINA (Verified Customer) (12-07-2021)

Good antibody, but sometimes it detects also aspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: lung and breast cancer cells

P glycoprotein Antibody Western Blot validation (1:1000 dilution) in lung and breast cancer cells (Cat no:22336-1-AP)

FH

Yu Siong (Verified Customer) (03-01-2019)

It worked perfectly for my porcine brain endothelial cells!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Porcine brain endothelial cells

P glycoprotein Antibody Western Blot, validation (1:500 dilution) in Porcine brain endothelial cells (Cat no:22336-1-AP)

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