ABCA2 Polyklonaler Antikörper

ABCA2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20681-1-AP

Synonyme

ABC transporter ABCA2, ABC2, ABCA2

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Galerie der Validierungsdaten

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  WB analysis of mouse thymus using 20681-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20681-1-AP (ABCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20681-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20681-1-AP (ABCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20681-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20681-1-AP (ABCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20681-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20681-1-AP (ABCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20681-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20681-1-AP (ABCA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, HepG2-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20681-1-AP bindet in WB, IHC, IF, ELISA ABCA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ATP-binding cassette, sub-family A (ABC1), member 2
• Berechnetes Molekulargewicht: 270 kDa
• Beobachtetes Molekulargewicht: 270 kDa
• GenBank-Zugangsnummer: NM_001606
• Gene symbol: ABCA2
• Gene ID (NCBI): 20
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ABCA2, also named as ABC2 and KIAA1062, belongs to the ABC transporter ABCA2, a member of the ATP-binding cassette (ABC) transporter family, is primarily expressed in brain and is involved in brain sterol homeostasis and associated with early onset Alzheimer's disease. ABCA2 is mainly localized in endosomes and lysosomes of oligodendrocytes and Schwann cells. It has also be reported as a marker of neural progenitor in the adult rodent brain. (PMID: 16539677)