AADACL1 Polyklonaler Antikörper

AADACL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14021-1-AP

Synonyme

EC:3.1.1.71, EC:3.1.1.-, Arylacetamide deacetylase-like 1, Acetylalkylglycerol acetylhydrolase, AADACL 1

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Galerie der Validierungsdaten

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  WB analysis using 14021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14021-1-AP (AADACL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14021-1-AP

  IP result of anti-AADACL1 (IP:14021-1-AP, 4ug; Detection:14021-1-AP 1:500) with COLO 320 cells lysate 1320 ug.

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  IHC staining of human brain using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 14021-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14021-1-AP (AADACL1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of COLO 320 using 14021-1-AP

  Immunofluorescent analysis of COLO 320 cells using 14021-1-AP (AADACL1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

14021-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA AADACL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AADACL1 fusion protein Ag5136
• Vollständiger Name: arylacetamide deacetylase-like 1
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC047588
• Gene symbol: AADACL1
• Gene ID (NCBI): 57552
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AADACL1(Arylacetamide deacetylase-like 1) is also named as NCEH1, KIAA1363 and belongs to the 'GDXG' lipolytic enzyme family. The transmembrane enzyme, AADACL1, controls the production of the monoalkylglycerol ether (MAGE) class of NELs in cancer cells and acts as a 2-acetyl MAGE hydrolase and is likely the principal source for this activity in tumor cells(PMID:21513884). The full length protein has three glycosylation sites and can be N-glycosylated(PMID:19159218). It has 3 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
rat	WB	J Proteomics; Integrative proteomics-metabolomics strategy reveals the mechanism of hepatotoxicity induced by Fructus Psoraleae.; Authors - Jingyi Duan
human	IHC	J Transl Med; Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer; Authors - Wei Tang
human	WB	Sci Rep; Lysophosphatidylglucoside/GPR55 signaling promotes foam cell formation in human M2c macrophages; Authors - Ryosuke Shimai