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Alpha 1B-Glycoprotein Polyklonaler Antikörper

Alpha 1B-Glycoprotein Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14181-1-AP

Synonyme

A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, Alpha 1B-Glycoprotein, GAB, HYST2477

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of human plasma using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of human plasma using 14181-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of BxPC-3 using 14181-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of MCF-7 using 14181-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    WB analysis of mouse kidney using 14181-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    IHC staining of human hepatocirrhosis using 14181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    IHC staining of human hepatocirrhosis using 14181-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14181-1-AP (Alpha 1B-Glycoprotein antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha 1B-Glycoprotein Polyclonal antibody (14181-1-AP)

    IF Staining of HepG2 using 14181-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14181-1-AP (alpha-1B-glycoprotein antibody) at dilution of 1:25 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Plasmagewebe, BxPC-3-Zellen, Mausnierengewebe, MCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Leberzirrhosegewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:20000
Immunhistochemie (IHC)IHC : 1:2500-1:10000
Immunfluoreszenz (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14181-1-AP bindet in WB, IHC, IF, ELISA Alpha 1B-Glycoprotein und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Alpha 1B-Glycoprotein fusion protein Ag5414
Vollständiger Name alpha-1-B glycoprotein
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht 65 kDa
GenBank-ZugangsnummerBC035719
Gene symbol A1BG
Gene ID (NCBI) 1
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

Protokolle

Produktspezifische Protokolle
WB protocol for Alpha 1B-Glycoprotein antibody 14181-1-APProtokoll herunterladen
IHC protocol for Alpha 1B-Glycoprotein antibody 14181-1-APProtokoll herunterladen
IF protocol for Alpha 1B-Glycoprotein antibody 14181-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen