4-Hydroxynonenal Monoklonaler Antikörper

4-Hydroxynonenal Monoklonal Antikörper für ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

4-Hydroxynonenal

Anwendung

, ELISA

Konjugation

Unkonjugiert

CloneNo.

2C1D10

Kat-Nr. : 68538-1-Ig

Synonyme

4-HNE, 4-Hydroxy-2-nonenal, 4-Hydroxynonenal, C9H16O2, Cas. 75899-68-2

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Galerie der Validierungsdaten

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  ELISA experiment of 4-Hydroxynonenal using 68538-1-Ig

  Indirect ELISA was performed by coating BSA conjugated 4-Hydroxynonenal (4-HNE) at ~20 ng/well (by 4-HNE amount), followed by blocking with 1% BSA. Serial diluted 4-Hydroxynonenal antibody 68538-1-Ig was added to the plates and incubated at 37℃. HRP-Goat anti-mouse was used for detection (top X-right Y, blue curve). Competitive ELISA was performed similarly except that different concentration of 4-Hydroxynonenal was mixed in 5 ng/mL primary antibody (bottom X-left Y, red curve).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in ELISA: 4-Hydroxynonenal

Empfohlene Verdünnung

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:5000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68538-1-Ig bindet in , ELISA 4-Hydroxynonenal und zeigt Reaktivität mit 4-Hydroxynonenal

• Getestete Reaktivität: 4-Hydroxynonenal
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTG
• Vollständiger Name: 4-Hydroxynonenal
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

4-Hydroxynonenal is a uremic toxin. Uremic toxins can be subdivided into three major groups based upon their chemical and physical characteristics: 1) small, water-soluble, non-protein-bound compounds, such as urea; 2) small, lipid-soluble and/or protein-bound compounds, such as the phenols and 3) larger so-called middle-molecules, such as beta2-microglobulin. 4-Hydroxynonenal (4-HNE) is a major aldehydic product of ω-6-unsaturated fatty acid peroxidation. It is considered a lipid peroxidation specific marker. 4-HNE has been found to induce differentiation and inhibit proliferation of HL-60 human leukemic cells. It has also been found to induce murine alveolar macrophage cell death. 4-HNE has been shown to inhibit State 3 respiration, causing a transient cytosolic Ca2+ increase. In addition, it irreversibly inhibits Na+-K+-ATPase activity.