SARS-CoV-2 Membrane Glycoprotein Polyklonaler Antikörper

SARS-CoV-2 Membrane Glycoprotein Polyklonal Antikörper für ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Virus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 28882-1-AP

Synonyme

2019-nCOV membrane glycoprotein, M protein, membrane glycoprotein

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  ELISA experiment of Recombinant protein using 28882-1-AP

  COVID-19 M Protein Antibody (28882-1-AP) tested by ELISA. COVID-19 M Protein(101-222aa ) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of COVID-19 M Protein Antibody (28882-1-AP). Bound antibodies were detected with HRP conjugated Goat anti-Rabbit IgG followed by incubation with HRP Substrate and teminated with H2SO4, then measuring the resulting absorbance at 450 nm.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in ELISA: Recombinant protein

Empfohlene Verdünnung

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

28882-1-AP bindet in WB, IF, ELISA SARS-CoV-2 Membrane Glycoprotein und zeigt Reaktivität mit Virus

• Getestete Reaktivität: Virus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SARS-CoV-2 Membrane Glycoprotein fusion protein Ag30691
• Vollständiger Name: COVID-19 M Protein
• GenBank-Zugangsnummer: NC_045512
• Gene symbol: SARS-CoV-2 Membrane Protein
• Gene ID (NCBI): 43740571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In the coronaviruses, the membrane (M) protein was reported to be the most abundant viral protein expressed during infection and a key protein in the assembly of both naked and enveloped virus particles (PMID: 11967315). The M glycoprotein is conserved across the β-coronaviruses. The multiple sequence alignment shows a remarkable similarity (98% identity) among the Sars-CoV-2 M variants and the sequences from Bat and Pangolin isolates (PMID:32596311). The M protein is predicted to contain a triple-spanning transmembrane (TM) region, a single N-glycosylation site near its N-terminus that is in the exterior of the virion, and a long C-terminal region in the interior (PMID: 15626342).

Publikationen

Species	Application	Title
	WB,IF	bioRxiv; Ubiquitin Ligase ITCH Regulates Life Cycle of SARS-CoV-2 Virus; Authors - Qiwang Xiang
		J Virol; Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation; Authors - Zhaohuan Wang